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Uba1 was PCR-amplified from cDNA and cloned into pGEX-4T1 vector, which introduced an N-terminal GST tag followed by a TEV cleavage site and a C-terminal His tag

Studies have shown that the activation of Wnt signaling in colorectal cancer is associated with the loss of function of the tumor regulator APC and the involvement of Wnt signaling has been reported in a variety of malignancies, including breast and stomach cancer (98,99)

Another allicin-derived molecule called allyl methyl sulfide is reasonably stable in blood, suggesting that the use of GO in the treatment of numerous infectious disorders (123)

Steinberg S & Kaplan IR (1984) The determination of low-molecular weight aldehydes in rain, fog and mist by reserved-phase liquid chromatography of the 2,4-dinitrophenyl-hydrazone derivatives